The biological pathways and markers identified as related to toxicity based on literature and integrated data analysis need to be confirmed with sound biological follow-up experiments.

This is a vital part of the project, which will provide additional data supporting the validity of the selected candidate safety biomarkers. This work will be carried out by the academic partners and will involve confirmation and validation of novel biomarkers of liver and kidney toxicity by state-of-the–art techniques both in vivo and in cell suitable cell culture systems in vitro.

The overall aim of this work-package is to enable the design of multiplex assays such as low density custom arrays or multiplex ELISA for rapid and sensitive detection of nephro- and hepatotoxicity in vivo and in vitro. An ancillary objective will be to utilize digital slide scanning technology to create an online image database of full face sections used for histological classification.